Passage of Wolbachia pipientis through Mutant Drosophila melanogaster Induces Phenotypic and Genomic Changes

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Passage of Wolbachia pipientis through mutant drosophila melanogaster induces phenotypic and genomic changes.

Wolbachia pipientis is a nearly ubiquitous, maternally transmitted bacterium that infects the germ line of insect hosts. Estimates are that Wolbachia infects 40 to 60% of insect species on the planet, making it one of the most prevalent infections on Earth. However, we know surprisingly little about the molecular mechanisms used by Wolbachia to infect its hosts. We passaged Wolbachia through no...

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Culture and phenotypic characterization of a Wolbachia pipientis isolate.

The recent isolation of Wolbachia pipientis in the continuous cell line Aa23, established from eggs of a strain of the Asian tiger mosquito Aedes albopictus, allowed us to perform extensive characterization of the isolate. Bacterial growth could be obtained in C6/36, another A. albopictus cell line, at 28 degrees C and in a human embryonic lung fibroblast monolayer at 28 and 37 degrees C, confi...

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Dynamics of Wolbachia pipientis Gene Expression Across the Drosophila melanogaster Life Cycle

Symbiotic interactions between microbes and their multicellular hosts have manifold biological consequences. To better understand how bacteria maintain symbiotic associations with animal hosts, we analyzed genome-wide gene expression for the endosymbiotic α-proteobacteria Wolbachia pipientis across the entire life cycle of Drosophila melanogaster. We found that the majority of Wolbachia genes a...

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Drosophila melanogaster mutant

M.L., ed.) 233-256; Claycomb, J.M., M. Benasutti, G. Bosco, D.D. Fenger, and T.L. Orr-Weaver 2004, Dev. Cell. 6(1): 145-55; Kramer, J.M., and B.E. Staveley 2003, Genet. Mol. Res. 2(1): 43-7; Liu, Y., and M. Lehmann 2008, Fly 2(2): 92-98; Manseau, L., A. Baradaran, D. Brower, A. Budhu, F. Elefant, H. Phan, A.V. Philip, M. Yang, D. Glover, K. Kaiser, K. Palter, and S. Selleck 1997, Dev. Dyn. 209(...

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Wolbachia Variants Induce Differential Protection to Viruses in Drosophila melanogaster: A Phenotypic and Phylogenomic Analysis

Wolbachia are intracellular bacterial symbionts that are able to protect various insect hosts from viral infections. This tripartite interaction was initially described in Drosophila melanogaster carrying wMel, its natural Wolbachia strain. wMel has been shown to be genetically polymorphic and there has been a recent change in variant frequencies in natural populations. We have compared the ant...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 2014

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.02987-14